摘要
目的为明确Survivin作为膀胱肿瘤患者的肿瘤诊断和监测标志物的可能性,我们检测了Survivin在膀胱肿瘤和非肿瘤患者病变组织的Survivin蛋白及其mRNA表达情况。方法从我科住院病人和膀胱镜检患者中获取31例膀胱癌患者(实验组)和24例非肿瘤患者(对照组)的组织标本,用免疫组织化学法(IHC)和RT-PCR检测Survivin蛋白和基因在肿瘤组织和非肿瘤组织中的表达情况。并对结果行相应的统计学分析。结果IHC检测实验组和对照组Survivin蛋白的阳性率分别为74%(23/31)和4%(1/24),χ2检验P<0.001,IHC的阳性率与肿瘤的分级分期有一定的关系;实验组和对照组RT-PCR检测Survivin mRNA的表达阳性率分别为90%(30/31)和4%(1/24),χ2检验P<0.001。结论膀胱肿瘤组织Survivin蛋白和基因的表达量高于对照组,因此,Survivin有望成为膀胱瘤高危人群筛选,肿瘤诊断和监测的工具之一。
Objective To elucidate the possibility of survivin as a marker of diagnosis and investigation of bladder tumor, the expressions of survivin protein and mRNA in bladder tumor and non tumor were tested. Methods The tissue of 31 cases tumor patients(experiment group) and 24 cases non tumor patients were gotten during operations and cystoscopy in our department. The survivin protein and mRNA transcription circumstance of all spectimen were test with immunohistochemical(IHC) and RT-PCR method, respectively. The results were analyzed by correspond statistic method. Results The positive rate of IHC of the experiment group and control group was 74%(23/31) and 4%(1/24), respectively.χ2 test P<0.001. Our study suggested that some relationship existed among the tumor stage, grade and the IHC positive rates. The positive rate of the survivin mRNA of experiment group and control group was 90%(30/31) and 4%(1/24),respectively.χ2 test P<0.001. Conclusion Our study suggested that the positive rate of surviving protein and mRNA was high in tumor than non tumor, So it was hopeful that survivin become one of bladder tumor markers as a tool of screening the high-risk goup of bladder tumor of diagnosing and investigating bladder tumor.
