DHA,EPA对耐力训练小鼠抗氧化能力影响的实验研究

Effect of DHA and EPA on mice with tolerance training in anti-oxidation

观察多不饱和脂肪酸DHA(二十二碳六烯酸)、EPA(二十碳五烯酸)对耐力训练小鼠抗氧化能力的影响。将小鼠随机分组,进行耐力训练后测定血浆和红细胞中脂质过氧化物丙二醛(MDA)的含量,肝脏、心肌、骨骼肌中MDA的含量、SOD(过氧化物歧化酶)活性及GPX(谷胱甘肽过氧化物酶)活性。训练结束即刻,训练组MDA含量、SOD活性及肝组织GPX活性显著升高;训练结束24h,训练组MDA含量、SOD活性及GPX活性降低。尤其对于饲加DHA,EPA鼠料组,MDA含量下降更为明显,其骨骼肌组织中SOD活性明显高于对照组。较大强度运动训练使机体内自由基生成增多,长期训练可增加机体清除自由基的能力,DHA,EPA有增加组织SOD活性和GPX活性的趋势。

To observe the anti-oxidation function of polyunsaturated fatty (DHA and EPA) on mice with tolerance training,the mice were divided randomly into three groups. After six weeks' training, mice were killed to examine the content of MDA in blood plasma, erythrocyte, liver, heart and quadriceps. The activities of SOD and GPX in liver, heart and quadriceps were also been determined. By the end of training, in two training groups the content of MDA, the activity of SOD in three tissues and the activity of GPX in liver were significantly improved. 24 hours later, the content of MDA in the two training groups were lower than ever, especially in the training experiment group (DHA and EPA group). The activity of SOD and GPX in three tissues in two training groups was lower. Compared with non-training control group, there was an increasing tendency. Training increased the free radicals and the capacity of eliminating free radicals. The tolerance training for a longer time increased the activity of SOD and GPX, especially in training experiment group (DHA and EPA group).