HPLC法对不同产地北豆根中蝙蝠葛碱的测定

Determination of Dauricine in Rhizoma Menispermi from different dist ricts by HPLC

目的 研究不同产地北豆根中蝙蝠葛碱含量的差异。方法 以北豆根饮片为分析对象,用phenomenexC18(4 . 6mm×15 0mm,5 μm)色谱柱,采用乙腈和0 . 2 %磷酸洗脱,流速1. 0ml·min-1,检测波长为2 84nm,对北豆根中的蝙蝠葛碱进行定性定量测定。结果 东北北豆根药材中生物碱含量最高,其次是山东产北豆根,河北产北豆根药材中蝙蝠葛碱含量较低。结论不同产地北豆根药材所含生物碱在质和量两方面均有较大差异。

Objective To study the difference of Daurici ne i n Rhizoma Menispermi from different districts.Methods HPLC an alysis method of Rhizoma Menispermi was studied and the chromatographic conditi on was as the following:phenom enex C 18(4.6mm×150mm,5μm)column;flow rate:1.0ml·min -1;mobi le phase:0.2%H 3PO 4 solution and CH 3CN;wavelength:284nm.Results The content of Dauricine in Rhi zo ma Menispermi from the North of China was highest,and the content of Dauricine in Rhizoma Menispermi from Hebei is low.Conclusion Considerable difference was detected among the several crude drugs from different districts.