哮喘患者痰液巨噬细胞炎症蛋白1α含量变化及意义

The changes of macrophage inflammatory protein-1 alpha(MIP-lα)level in sputum of asthmatic patients and their clinc significance

目的探讨痰液中巨噬细胞炎症蛋白1α(MIP-1α) 水平与哮喘严重程度的关系。方法采用双夹心抗体酶联免疫吸附法检测39例不同时期、不同程度哮喘患者痰液MIP-1α水平, 同步测定哮喘患者嗜酸细胞阳离子蛋白(ECP) 含量及肺通气功能(PEV1 占预计值% )。结果哮喘轻、中、重度发作期患者痰液MIP-1α水平与缓解期及正常对照组比较,差异具显著性(P<0. 05); 发作期与缓解期比较, 差异具显著性(P<0. 05)。哮喘缓解期及发作期患者痰液中MIP-1α与FEV1 占预计值%呈显著负相关, 相关系数分别为(r=-0 539, -0 447, P均<0 05), 而痰液中MIP-1α与ECP之间呈显著正相关(r值分别为0 541, 0 527, P<0 05)。13例中、重度哮喘患者治疗后, 痰液MIP-1α浓度与治疗前比较差异有显著性(P<0 05)。结论痰液MIP-1α参与了哮喘急性发作并反映哮喘的严重程度, 评价药物疗效的指标。

Objective To explore relation between the level of macrophage inflammatoryprotein-1 alpha(UlP-la) in the sputum and the severity of bronchial asthma. Method 39 patients with asthma at remission(group I,n=13)orexacerbation state with mild attack(group Ⅱ,n=13)or moderate to severe attack(group Ⅲ,n=13),normal control subjects(group IV,n=15)were included in this study. MIP-1 α were measured by enzyme link immunoabsorbent assay, while the eosinophil cational protein(ECP) and pulmonary function were also measured in tested patients with asthma. Results The levels of MIP-1 α in patients with asthma were significantly higher than that in controls;those of group Ⅱ and group Ⅲ were significantly higher than that of groupI,The level of MIP-lα derivatives in sputum was closed correlated with the degree of airflow obstruction and sputum ECP levels in patients with asthma at remission (r=-0.593, P<0.05;r=0.541, P<0.05 respectively)and exacerbation(r=-0.447, P<0.05;r=0.527, P<0.05 respectively).A significant decrease in concentration of sputum MIP-1 α was observed after therapeutic treatment of patients with moderate to severe attack. Conclusion The level of MIP-lα in sputum may beausefulmarkerformonitoringthestateofAirway inflammation and participating in the exacerbation of asthma and Assessing the therapeutic efficacy.