心肺复苏犬心肌及血浆内皮素含量变化的实验研究

Changes of myocardial and plasma endothelin(ET) in cardiopulmonary resuscitation dogs

目的:研究复苏犬心肌组织及血浆内皮素(endothelin,E T)含量的变化规律及意义。方法:电击致犬室颤、心跳呼吸骤停,15m in后进行标准复苏,观察心脏停跳15m in、复苏后0.5h、2h、4h时心肌组织及血E T含量。结果:复苏后0.5h心肌组织及血浆E T含量较对照组明显升高(P<0.01),复苏后2h血浆E T含量达最高,心肌组织E T含量持续升高至复苏后4h(P<0.01)。结论:复苏后心肌组织及血中E T含量变化可能是机体在急剧缺血再灌注后出现的一种强烈的自身应激调节机制,与复苏后的心肌损伤及血流动力学不稳定有关。

Objective To observe the changes of endothelin(ET) contents of myocardium and plasma in resuscitation dogs. Methods All dogs were given electrical shock with ventricular fibrillation/cardiac arrest(CA) and normally resuscitated after 15 min. ET contents of myocardium and plasma were measured at 15 min after CA and 0.5 h,2 h,4 h after cardio pulmonary resuscitation(CRP),respectively. Results Compared with that of the control group,ET contents of myocardium and plasma increased markedly at 0.5 h(P< 0.01) and reached to the peak value at 4 h and 2 h after CRP(P< 0.01 and < 0.01). Conclusion Significantly increased ET contents of myocardium and plasma after CPR in dogs could be a intense stress regulating mechanism after cardiac ischemia reperfusion and could be one of important factors leading to myocardial injury and hemodynamic instability.