期刊文献+

丙型肝炎病毒IgG抗体阳性患者血清抗HCV IgM、ALT及HCV RNA的对比分析 被引量:3

下载PDF
导出
摘要 目的:探讨丙型肝炎(H C V)IgG阳性患者的血清抗-H C V IgM、丙氨酸转移酶(A LT)水平与H C V R N A之间的关系。方法:通过第三代E IA试剂盒检测抗-H C V IgG、抗-H C V IgM,全自动生化分析仪检测丙型肝炎患者A LT水平,荧光定量逆转录聚合酶链反应(R T-PC R)方法检测H C V R N A,并进行比较。结果:在丙型肝炎IgG阳性患者中,抗-H C V IgM阳性率为8.3%,H C V R N A阳性率为41.7%。H C V R N A的检出在A LT异常情况下较正常明显高(P<0.05),但A LT水平与H C V R N A含量无线性相关性(r=0.346,P>0.05)。结论:抗-H C V IgM不能反映病毒复制,A LT异常情况下H C V R N A检出率高,但A LT水平与H C V R N A含量无线性相关性,H C V R N A仍是反映H C V复制的可靠指标。
出处 《实用医学杂志》 CAS 2005年第7期745-746,共2页 The Journal of Practical Medicine
  • 相关文献

参考文献11

  • 1闵福援,吴昊,王健,林秀玉,汪俊韬.用ELISA法检测丙型肝炎病毒IgM抗体试剂盒的研究[J].中华实验和临床病毒学杂志,1994,8(2):158-160. 被引量:12
  • 2程钢,何蕴韶,周新宇,李虎.丙型肝炎病毒(HCV)荧光PCR(F-PCR)试剂盒的研制及与免疫学方法的比较[J].中国免疫学杂志,2002,18(7):464-468. 被引量:10
  • 3opes EP,Granato CH,Lanzoni V,et al. Evaluation of an enzyme immunoassay for hepatitis C virus antibody detection using a recombinant protein derived from the core region of hepatitis C virus genome. Mem Inst Oswaldo Cruz,2000,95(5): 717-720.
  • 4Sarrazin C. Highly sensitive hepatitis C virus RNA detection methods: molecular backgrounds and clinical significance. J Clin Virol,2002,25(suppl 3): 523-529.
  • 5Zaaijer HL,Mimms LT,Cuypers HT,et al. Variability of the IgM response in hepatitis C virus infection. J Med Virol,1993,40(3): 184-187.
  • 6Brillanti S,Foli M,Perini P,et al. Long-term persistence of IgM antibodies to HCV in chronic hepatitis C. J Hepatol,1993,19(1): 185-186.
  • 7Mancini C,Rivanera D,LiLLi D,et al. IgM anti-HCV in patients with chronic non-A-non-B hepatitis and their relationship to viral replication. Clin Diagn Virol,1995,4(4): 293-299.
  • 8Martinelli AL,Brown D,Braun HB,et al. Quantitative assessment of C virus RNA and IgM antibody to hepatitis C core in chronic hepatitis C. J Hepatol,1996,24(1): 21-26.
  • 9Abe K,Inchauspe G,Shikata T,et al. Three different patterns of hepatitis C virus infection of chimpanzees. Hepatology,1992,15(4): 690-695.
  • 10Casino C,Lilli D,Rivanera D,et al. Diagnostic value of anti-Hepatitis C virus(HCV) Core immuoglobulin M in reccurrence of HCV infection after orthotopic liver transplantation. J Clin Microbiol,1999,37(8): 2726-2728.

二级参考文献5

  • 1Fagan E A,Guamer P,Perera S D et al.Quantitation of hepatitis B virus DNA(HBV DN) in serum using the spot hybridization technique and scintillation counting[].Journal of Virological Methods.1985
  • 2Holland P M,Abramson R D,Watson R et al.Detection of specific poly-merase chain reaction product by utilizing the 5’ to 3’ exonuclease activity of the Thermus aquaticus DNA polymerase[].Proceedings of the National Academy of Sciences of the United States of America.1991
  • 3Livak K J,Flood S J A,Marmaro J et al.Oligonucletides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization[].PCR Methods and Applications.1995
  • 4Jalava T,Lehtovaara P,Kallio A et al.Quantification of hepatitis B virus DNA by competitive amplification and hybridization and hybridization on microplates[].Biotechniques.1993
  • 5Nolte F S,Thurmond C,Fried M W.Preclinical evaluation of AMPLICOR hepatitis C virus test for detection of hepatits C virus RNA[].Journal of Clinical Microbiology.1995

共引文献20

同被引文献31

引证文献3

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部