维拉帕米对脑膜瘤生长影响的实验研究(英文)

Study of effects of Verapamil on growth of meningioma

目的探讨钙通道阻滞剂维拉帕米对脑膜瘤细胞增殖的影响。方法将不同浓度的维拉帕米作用于培养的脑膜瘤细胞,MTT法观察其对细胞增殖的抑制作用。建立裸鼠皮下脑膜瘤模型,观察服用维拉帕米后肿瘤的生长情况,免疫组化检测皮下肿瘤增殖细胞核抗原(PCNA)的表达。结果维拉帕米浓度呈依赖性地抑制脑膜瘤细胞增殖,浓度为1μmol/L时即有抑制效应,100μmol/L时抑制作用最明显。服药组肿瘤的体积明显小于对照组(P<0.01),其PCNA的表达也低于对照组(P<0.05)。结论维拉帕米在体外和体内均可抑制脑膜瘤细胞的增殖和生长。

To investigate the effects of verapamil on the proliferation of meningioma cells in vitro and in vivo. Verapamil with various concentration were put in the cultured meningioma cells and after 24 hours by means of MTT to value the proliferation of meningioma cells. The meningioma model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups. One group was treated with verapamil resolved in water and the other is a control group without verapamil. Tumors volume was measured and after 12 weeks the tumors were taken out and examined histologically. Proliferating cell nuclear antigen (PCNA) was detected with immunohistochemistry. Verapamil inhibits the growth of cultured meningioma cells in concentration dependant. The inhibition can be observed in the concentration of 1 μmol/L and the mostly obvious effects appear in the concentration of 100 μmol/L. Tumors volume of the verapamil treated group is obviously smaller than that of the control group(211.40±5.50 vs 163.94±3.62, P <0.01)and its expression of PCNA is also lower(1.52±0.24 vs 2.86±0.53). Tumor inhibition rate is about 22.45%. [Conclusion] Verapamil can inhibit the proliferation of meningioma cells in vitro and vivo.