摘要
目的研究依贝沙坦对心肌梗死后大鼠心肌Smad表达及心室重构的影响。方法冠状动脉结扎建立大鼠心肌梗死(MI)模型,24 h后存活大鼠随机分为安慰剂组和依贝沙坦组,另设假手术组。8 周后,测量心室重量/体重(HW/BW),非梗死区胶原含量,梗死区、非梗死区心肌转化生长因子(TGF)β1mRNA、Smad3 mRNA的表达,多普勒超声评价心脏功能。结果安慰剂组与假手术组比较HW/BW、左心室舒张末期内径(LVDd)、E/A比值、非梗死区胶原含量、梗死区及非梗死区TGFβ1 mRNA、Smad3 mRNA的表达均增加;射血分数(EF)、短轴缩短率(FS)、后壁(PW)增厚率均降低。依贝沙坦组与安慰剂组比较,上述指标显著改善。结论TGF-β-Smad传导通路可能参与MI后的心室重构,依贝沙坦可抑制Smad表达并减轻MI后左室重构。
Objective To evaluate the effects of irbesartan on Smad3 expression and ventricular remodeling after myocardial infarction(MI).Methods MI was induced by ligating the left anterior descending coronary artery of the rats.Twenty-four hours after ligation ,the survival rats were randomly divided into two groups and treated for 8 weeks .①MI+placebo;②MI+ irbesartan (50 mg·kg^-1 ·d^-1 );sham-operated rats were used as normal control.8 weeks later,we examined heart weight/ body weight ratio(HW/BW),left ventricular end-diastolic dimension (LVDd) ,ejection fraction (EF),fractional shortening (FS),E-wave/A-wave velocity ratio,posterior wall(PW) thickening by echocardiography;collagen content; the expression of transforming growth factor (TGF)β_1mRNA,Smad3 mRNA in the infarcted and un-infarcted area by RT-PCR.Results HW/BW(P<0.01),LVDd(P<0.01),E/A(P<0.01),collagen content(P<0.01),the level of TGF β_1 mRNA and Smad3 mRNA(P<0.01) in place bo-treated infarcted rats increased significantly, compared with sham-operated rats.Irbesartan limited the increase compared with placebo-treated infarcted rats (P<0.01).Compared with sham-operated rats, EF(P<0.01),FS(P<0.01),PW thickening(P<0.01) significantly decreased placebo-treated infarcted rats.Irbesartan improved the decrease compared with placebo-treated infarcted rats (P<0.01).Conclusion These results indicated that TGF β_1-Smads signaling were correlated with the ventricular remodeling after myocardial infarction.Irbesartan could inhibit Smad expression and prevent the ventricular remodeling . [
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第4期260-262,共3页
Chinese Journal of Critical Care Medicine
基金
黑龙江省自然基金资助项目(No.D0239)
