加速性脑创伤大鼠皮层和纹状体含水量的动态变化

Dynamic change of brain water content in the area of cortex and striatum after traumatic brain injury by impact-acceleration on rats

目的探讨加速性脑创伤后,大鼠皮层与纹状体组织含水量的动态变化。方法采用改良的大鼠加速性脑创伤模型,利用组织比重法测定创伤后不同时间局部脑组织含水量的变化。结果改良的大鼠加速性脑创伤模型的死亡率为46.4%,而颅骨骨折的发生率从12.5%下降至7.1%。创伤后24 h内,存活大鼠皮层和纹状体组织含水量呈现先略下降后升高的变化趋势。尽管24 h大鼠皮层组织含水量明显高于8 h组大鼠,但其绝对值仅升高0.4%。结论颅脑创伤后无明显颅内占位病变和呼吸、循环变化的大鼠,24 h内其脑水肿轻微。

Objective This research is to investigate dynamic change of brain water content (BWC) in the area of cortex and striatum after traumatic brain injury by means of impact-acceleration on rats. Method By use of the model improved by impact-acceleration in rats, the regional brain water content was measured by means of gravimetric technique at different time points after traumatic brain injury. Result The mortality of the improved model of traumatic brain injury was 46.4%, but the rate of skull fracture was decreased from 12.5% to 7.1%. Within the first 24 hours after the traumatic brain injury, the BWC of cortex and striatum in the survival rats showed, at first, a slight decrease, and then an increase. The BWC in the area of cortex at the time of 24 hours after impact-acceleration was increased only 0.4% in comparison with that at the time of 8 hours following the traumatic brain injury, although this change was significant in terms of statistics. Conclusion The BWC of the survival rats without evident mass effect and cardio-respiration disorder has a slight change after 24 hours. [