摘要
为Dx5基因设计1对特异引物,利用这1对引物,对小偃22×738组合中的BC2、BC2 、F2、F3和F4代的Dx5基因进行了PCR检测。结果表明,凡是携带Dx5基因的材料均扩增出一条450bp长度的特异条带,而未携带该基因的材料则不能扩增出这一特异条带。研究中还发现,对检测优质面包烘拷品质而言,PCR技术是最简便、准确、快速的方法之一。
The Dx5 gene of the BC_2 , BC_2, F_2, F_3 and F_4 generations in the 22×738 group of Xiaoyan was detected by (designing) a pair of specific primers. The results show that any material that carries the Dx5 gene amplificatesou a specific band of 450bp length, while the material that doesn’t carry the Dx5 gene can not amplificate this specific band. It is also foud that PCR (technology) is one of the most convenient, accurate and simplest methods as to detecting the baking quality of high-grade bread.
出处
《贵州农业科学》
CAS
2005年第2期17-18,共2页
Guizhou Agricultural Sciences
基金
陕西省杨凌农业生物技术育种中心项目(2001B 5)
