肾素血管紧张素系统、内皮素及降钙素基因相关肽在肾性高血压大鼠的动态变化

THE CHANGES OF RAS,ET AND CGRP IN RENOVASCULAR HYPERTENSIVE RATS

目的 探讨肾素血管紧张素系统、内皮素及降钙素基因相关肽在肾性高血压大鼠中的作用和意义。方法 制造肾性高血压大鼠模型,无创尾套法测量记录鼠尾动脉收缩压,采用放射免疫分析的方法测量所收集的血浆中肾素活性水平 (PRA),以及血管紧张素Ⅱ (AngⅡ )、醛固酮(Ald)、内皮素(ET)及降钙素基因相关肽(CGRP)的含量。结果 肾动脉狭窄使血压及血浆中PRA、AngⅡ、Ald、ET的含量有不同程度的升高,而血浆中CGRP含量则下降,与同期假手术组比较差异有显著性 (P<0. 05)。结论 肾素血管紧张素系统、内皮素和降钙素基因相关肽在肾性高血压的发生发展中起重要作用。

Objective To study the effect of Renin-Angiotensin-Aldosterone system (RAS), Endothelin (ET), Calcitonin gene-related peptide (CGRP) in renovascular hypertensive rats. Methods Two-Kidney, one-clip renovascular hypertension rat (2K1C-RHT) was established. Systolic blood pressure (SBP) was measured. Serum concerntretions of rennin activity (PRA), angiotension II (AngII).Aldosterone(Ald), CGRP and ET were measured in each group by radioimmunoassay method, respectively. Results The blood pressure and concentrations of ET, PRA, AngII and Ald in plasma were increased, while the concentration of CGRP was decreased in plasma in the hypertension group. A significant statistical difference was observed between hypertension group and sham operated group. Conclusion RAS, ET, CGRP were positively correlated with renovascular hypertension.