云芝胞内多糖CVP-Ⅱ的分离与初步鉴定

ISOLATION AND STRUCTURE IDENTIFICATION OF CVP-Ⅱ, THE INTRACELLULAR POLYSACCHARIDE OF CORIOLUS VERSICOLOR

从液体发酵的云芝菌丝体中提取获得菌丝体胞内多糖,,经DEAE-SepharoseCL-6B洗脱分离出一种主要组分CVP-Ⅱ,该组分经凝胶层析显现单一峰,初步鉴定CVP-Ⅱ为较纯组分,对CVP-Ⅱ进行紫外分析,结果发现该组分呈现蛋白结合多糖的特征,从红外分析来看,明显呈现出多糖的官能团吸收.

The intracellular polysaccharide extracted by hot-water from the mycelium of coriolus versicolor was separated by DEAE Sepharose CL-6B,and the main component CVP-Ⅱwas collected from the effluent. Its homogeneity were confirmed by Sepharose CL-6B chromatography. The UV spectrum indicated that the CVP-Ⅱ belongs to proteoglycan, and its FT-IR spectrum showed the characteristics of polysaccharide group from FI-IR spectrum.