用双单倍体群体构建水稻的分子连锁图

Construction of a Rice Molecular Linkage Map Using a Double Hploid(DH)Population

本研究以窄叶青８号（籼稻）×京系１７（粳稻）的Ｆ１花培株系──ＤＨ群体为基础建立了１个水稻的ＲＦＬＰ连锁框架图，该图含ＲＦＬＰ标记、同功酶标记等共１０８个位点，标记间的平均间距为８．６ｃＭ。该图谱与已发表的用其他群体构建的图谱有很高的可比性。利用该框架图定位了２个未知位点的同功酶标记基因和１个籼稻亲本的抗稻瘟病基因。研究表明，目前的群体可进一步扩大成为一个永久性的作图群体。

In this research,a rice molecular map was constructed using a DH population from ZYQ8(indica)/JX17(japonica).It consists of 108 loci including RFLP and isozyme markers.The mean map distance between markers is 8.6cM.This map bears comparison with the other published rice maps.Two unknown isozyme loci and one rice blast resistance gene from ZYQ8 were located on this map.This research revealed that the current population can be further enlarged and become a permanent mapping population for gene location and genome study.