人体乳腺癌细胞系Bcap－37和MCF－7的细胞遗传学研究

Cytogenetic Studies of Human Breast Cancer Cell Lines Bcap-37 and MCF-7

应用低温同步法与秋水酰胺处理，对人体乳腺癌细胞系Ｂｃａｐ－３７和ＭＣＦ－７的中期及早中期细胞进行Ｇ－显带分析。研究表明，Ｂｃａｐ－３７细胞染色体众数为６３，可识别其结构的标记染色体１７条；ＭＣＦ－７细胞染色体众数为５６，可识别其结构的标记染色体１３条。结合文献报道以及本研究结果显示，乳腺癌中最常涉及到第１、３、５、７、１１、１３和１７号染色体结构及数目的异常，染色体断裂点１ｐ１１（１ｑ１１）、１ｐ１３、３ｐ２１、３ｑ１１、５ｑ１１、６ｑ１３、６ｑ２３、７ｑ２２、１１ｐ１３和１１ｐ１５也经常涉及；它们可能与癌相关基因的激活和抗癌基因的丢失有关，从而在乳腺癌发生发展中起一定作用。

The cells of human breast cancer cell lines Bcap-37 and MCF-7 were treated with low temperature and colchichine, G-banded metaphases and early-metaphases were analysed. The resutls demonstrated extensive aneuploidy with both numerical and structural abnormalities in both cell lines. It was found that Bcap 37 cell line had 63 chromosomes with 17 identified markers in most examined cells. The modal chromosome numbers in MCF-7 cell line was 56, and 13 identified markers were found in MCF-7. The results suggested that the numerical and structral changes of chromosome 1,3,5,7,11,13, and 17 might be associated with breast cancer, and that the breakpoints 1p11(1q11), 1p13. 3p21, 3q11, 5q11, 6q13, 6q23, 7q22, 11p13 and 11p15 might be significant in the genesis of breast cancer, which maybe related to the expression of activated oncogenes and the loss of antioncogenes.