P53、P21^(WAF1)、CyclinE与乳腺癌临床病理指标的关系

Expression and Significance of P53,P21^(WAF1) ,Cyclin E in Breast Carcinoma

目的:通过检测P53蛋白、P21WAF1蛋白、CyclinE在乳腺癌中的表达及其与临床病理指标的关系,探讨乳腺癌的生物学特性,分析它们在乳腺癌的发生、转移中所起的作用。方法:应用免疫组化S-P法检测 88例乳腺癌石蜡包埋标本中P53、P21WAF1、CyclinE的表达情况。结果:有淋巴结转移组P53的阳性表达率 76. 92%高于无淋巴结转移组的阳性率 36. 73%。淋巴结转移组P21WAF1的阳性表达率 23. 08%明显低于无淋巴结转移组的阳性率46. 94%。CyclinE在乳腺癌Ⅱ~Ⅲ级中阳性表达率 55. 07%明显高于Ⅰ级中阳性表达率 26. 32%, 有淋巴结转移组CyclinE的阳性表达率71. 79%高于无淋巴结转移组的阳性率 30. 61%。结论:P53、P21WAF1、CyclinE与乳腺癌的发生、发展有关,可作为判断患者淋巴结转移及推断预后的指标。

Objective:To investigate the biological features of breast carcinoma and analyze P53,P21 WAF1 and Cyclin E 's functions in the genesis,progression and metastasis of breast carcinoma by the examination of P73,P21 WAF1 and Cyclin E,and their relationship with clinical-pathological feature indexes.Methods: A total of 88 paraffin-embedded tissue preparations of breast carcinomas were observed by the use of immunohistochemical S-P method to examine the expression of proteins of P53,P21 WAFI and Cyclin E.Results:The postive expression rate of P53 in the group with lymph nodes metastasis was 76.92%, that of P53 in non-lymph nodes metastasis was36.73%. the former was higher than the latter, with significant difference.The positive rate in the group with lymph nodes metastasis was 23.08%,which was remarkably lower than that (46.94%)of non-lymph nodes metastasis.The difference was significant. The positive expression rate (55.07%) of Cyclin E in Grade Ⅱand Ⅲ cancer was higher than that(26.32% ) of Cyclin E in Grade Ⅰ,with significant difference. The positive rate of Cyclin E in the group with lymph nodes metastasis was 71.79%,which was higher than that(30.61%) of non-lymph nodes metastasis.The difference was significant. Conclusion: It revealed that P53 , P21 WAF1 and Cyclin E were correlated with the genesis and progression of breast carcinomas . The findings suggested that the expression of them might be a useful marker for predicting the lymph node metastasis, and, further, the prognosis of breast carcinoma.