银屑病患者血清TNFα浓度及其在皮损中的表达研究

The study of TNF-α in sera and its expression in skin lesions of patients with psoriasis

目的:评价TNF- α及其受体在寻常型银屑病血清中水平及组织中的分布及其在病理机制中的作用。方法:应用双抗体夹心ELISA方法及免疫组化检查检测16例寻常型银屑病患者及10例正常人血清中TNF -α浓度和皮肤标本中TNF -α及其受体的分布。结果:正常人与银屑病患者血清TNF -α的浓度分别为: ( 1. 823±4. 133 )pg/ml和(83. 643±110. 104)pg/ml,P=0. 02849。16例患者Z12抗体及抗p55受体抗体组化结果均为阳性,其广泛表达于表皮全层及真皮浅层; 10例正常人中Z12抗体阳性者2例,抗p55受体抗体阳性者5例,呈散在、少量分布于表皮基底层及真皮浅层。结论:TNF-α水平在寻常型银屑病患者血清及皮肤组织中均高于正常人。

Objective:To evaluate TNF-α and its receptor in sera and in skin lesions with psoriatic patients, and find the way which TNF-α exert its role on the mechanism of pathology of psoriasis vulgaris. Methods:We used ELISA and immunohistochemical method to check TNF-α and its receptor in sera and in lesions of 16 patients and 10 healthy volunteers. Results:The concentrations of TNF-α in sera of healthy volunteers and patients were 1.823±4.133 pg/ml and 83.643±110.104 pg/ml,respectively,P=0.02849. All 16 patients were positive stained by Z12 antibody and anti-p55 TNF-R antibody, and their expressions throughout the epidermis and the upper dermis. Only 2 and 5 healthy volunteers were positive stained by Z12 antibody and anti-p55 TNF-R antibody respectively, and their expressions were scattered to the basal cell layers of the epidermis and the upper dermis. Conclusions:TNF-α were elevated in sera and skin lesions of psoriatic patients compared with that of healthy volunteers.