视黄醇对人淋巴细胞抗体生成能力的促进作用

AUGMENTATIVE EFFECT OF RETINOL ON IMMUNOGLOBULIN SYNTHESIS OF HUMAN LYMPHOCYTES

将新生儿脐带血和成人静脉血中淋巴细胞与不同浓度的视黄醇进行体外培养试验，通过对培养液中免疫球蛋白含量的测定观察维生素Ａ对淋巴细胞抗体生成能力的影响。结果表明，对于自脐血中分离的淋巴细胞，视黄醇的最佳效应浓度为１×１０￣（－６）ｍｏｌ／Ｌ，该浓度恰是人体血清的生理水平，在此条件下淋巴细胞产生的ＩｇＭ水平高于无视黄醇培养对照纽约５．９倍。相反，自成人血中分离的淋巴细胞在视黄醇浓度低为１×１０（－１０）至１０（－１４）ｍｏｌ／Ｌ时表现出ＩｇＧ的产量增加，提示正常血清水平的维生素Ａ对维持和促进小儿淋巴细胞对外来性抗原的反应有非常重要的影响。

unonuclear cells from cord blood (CBMC) or adult peripheral bllod(PBMC)were incubated with retinol(ROH), immunoglobulins concentra-tion in the culture were assayed to determine the effect of ROH on teh function fo lymphocytes. The results showed that production of Ig in te culture with ROH was 5. 9 times higher than that in the ROH-free control, the optimal responsible concentration of ROH was 1×10(-8)mol/L, similar to the physical level of human serum. In contrast, the augmentating effect of ROH on PBMC demonstrated a lower response, the optimal res-ponse concentration was 1×10(-13)-1× 10(14)mol/L. Our study suggested that vitamin A nutritional status is important to maintain lymphocytes func-tion of young infants.