高效液相色谱法同时测定强化食品中的维生素A、D、E

THE SIMULTANEOUS DETERMINATION OF VITAMIN A,VITAMIN D AND VITAMIN E IN FORTIFIED FOODS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

用反相高饮液相色谱法同时测定强化食品中维生素Ａ、维生素Ｄ和维生素Ｅ的含量。对不同成分的样品采用不同的提取步骤。测定回收率分别为９２．１８％～９８．７２％、９１．６１％～９７．７８％和９２．００％～９７．２５％。变异系数（ＣＶ）分别为１．０３％～５．２３％、２．３７％～５．３３％和２．６７％～３．７２％。

itamins A,D and E in fortified foods Were determined by reversepliase high performance liquid chromatographic method(RPLC),Two extrac-tion steps were used for samples of different components.The recoveries forvitamins A,D and E were 92.18%一98.72%, 91.61%-97.78%and 92.00%一97.25%respectively. The coefficients of variation(CV)for the determina一tion were 1.03%一5.23%,2.37%一5.33%and 2.67%一3.72%respectively.