通因通用法治疗溃疡性结肠炎的临床及实验研究

Clinical and experimental research of method of treating diarrhea with purgatives in treatment of ulcerous colonitis

[目的] 观察调肠通络饮(TTD)对溃疡性结肠炎(UC) 患者的疗效及对实验性UC 大鼠血小板功能的影响。[方法] 103例UC患者随机分为两组,分别予TTD、柳氮磺胺砒啶(SASP)治疗后,观察临床综合疗效与结肠黏膜病变疗效;以2,4,6三硝基苯磺酸(TNBS)诱导实验性大鼠结肠炎,分别予TTD、SASP治疗后腹主动脉采血,测血小板黏附率、血小板聚集性,血浆与结肠组织中血栓烷B2 (TXB2)、6 酮前列腺素F1a(125 Ⅰ6 -Keto -PGF1α),并取结肠组织,作病理观察。[结果] TTD组综合疗效明显优于SASP组;TTD能显著改善UC 大鼠结肠病理状态,降低血浆及结肠组织中TXB2,升高血浆6- Keto -PGF1α。[结论] TTD为治疗UC的有效方药,能改善UC大鼠病损状况,有抑制血小板功能亢进的作用。

Objective] To observe the curative effect of the method of treating diarrhea with purgatives on the patients of ulcerous colonitis (UC) and on the function of blood platelet of rats with experimental UC.[Methods] One hundred and three patients with UC were randomly divided into 2 groups which were given Tiaochang Tongluo decoction (TTD) and SASP respectively. After the treatment, clinical comprehensive curative effects and the effect on pathological colonic membrane were observed. TNBS was used to induce UC in rats, which were given TTD and SASP respectively. After the treatment, blood in the aorta of the belly was taken to test adhesion rate and collection of blood platelet. TXB_2、 125 I-6-Keto-PGF_ 1α in the blood plasma and colonic tissue were measured. Pathological changes of colon were examined.[Results] The comprehensive curative effect of the TTD group was obviously better than that of the SASP group .TTD remarkably improved the pathological condition in the colon of UC rats, reduced the contents of TXB_2 in the blood plasma and colonic tissue and raised the level of 6-Keto-PGF_ 1α in the blood plasma.[Conclusion] TTD, an effective formula for treating UC, can improve the pathological injury in the colon of the UC rats and inhibit the hyperfunction of blood platelet.