药根碱、小檗碱、黄连煎剂及模拟方对小鼠血糖的影响

Effect of jatrorrhizine, berberine, Huanglian Decoction and compound-mimic prescription on blood glucose in mice

目的研究药根碱(Jat)的降血糖作用,并比较Jat、小檗碱(Ber)、黄连煎剂(HLD)及模拟方(Ber+Jat)对小鼠血糖水平的影响。方法采用薄层扫描法测定HLD中Ber和Jat的质量分数,并根据其比值配制模拟方。小鼠ip四氧嘧啶制备糖尿病模型,模型小鼠和正常小鼠均ig给予不同剂量Jat、Ber、HLD和模拟方,葡萄糖氧化酶法测定血糖。结果Jat、Ber、HLD和模拟方均有不同程度的降低糖尿病和正常小鼠血糖的作用,HLD作用最强,Jat较同剂量Ber作用弱,模拟方与单用Ber作用相当。结论黄连中可能还有除Ber和Jat之外的降血糖成分,以天然成分比例配伍Ber和Jat,未见明显协同或拮抗作用。

Objective To study the hypoglycemic activity of jatrorrhizine (Jat) and compare the effects of Jat, berberine (Ber), Huanglian Decoction (HLD), and compounds-mimic prescription (Ber+Jat) on blood glucose level in mice. Methods TLC-scanning method was adopted for the determination of Ber and Jat in HLD. Ber+Jat was made artificially according to the result of the TLC-scanning. Diabetic model mice were made by ip alloxan, diabetic and normal mice were given Jat, Ber, HLD, and Ber+Jat in different dose by ig and applied in the study. Blood glucose level was analyzed by spectrophotometry with glucose-oxidase. Results Jat, Ber, HLD, and Ber+Jat decreased blood glucose level of diabetic and normal mice at different degrees. HLD showed the most significant hypoglycemic activity. Jat also possessed the function of decreasing blood glucose, which was less than that of Ber at the same dose. There was no significant difference between Ber+Jat and Ber. Conclusion It implies that there are some other hypoglycemic ingredients in HLD besides Ber and Jat. Jat has no evident synergistic or antagonistic action when coexisted with Ber in natural ratio.