荒漠肉苁蓉茎腐病的初步研究

Stem rot of Cistanche deserticola

目的分离和鉴定肉苁蓉茎腐病病原菌,研究其生物学特性,并室内筛选对该病原菌有效的杀菌剂。方法组织分离法和菌丝尖端纯化法得到主要病原菌,通过测量不同营养条件下菌落生长直径研究其生物学特性,平皿菌丝生长抑制法进行杀菌剂的生物测定。结果接骨木镰刀菌Fusariumsambucinum是肉苁蓉茎腐病的主要病原菌,为首次记录;适合其生长的培养基为PSA培养基,对碳和氮的吸收主要以蔗糖和蛋白胨为主,适宜生长温度为10～30℃,适宜生长pH为6～8;多菌灵、菌线威、绿享二号在室内可以有效地抑制病原菌。结论该病原菌的分离鉴定可以为该病害原的防治提供理论依据。

Objective To isolate and identify pathogen of Cistanche deserticola stem rot, study its biological characteristics, and select the effective fungicides against the pathogenic bacteria.Methods The main pathogen can be gained by tissue isolation and mycelium cusp purification, the biological characteristics can be studied by measuring the growth diameter under different nutrient conditions and seeded the hyphae disk at the centre of agar plate containing chemicals to do fungicide bioassay.Results Fusarium sambucinum was the main pathogenic bacteria on C.deserticola stem rot which was first recorded; its suitable medium was PSA culture medium; among the carbon and nitrogen sources supplied, saccharose and peptone were most available; the temperature range of its growth was 10-30 ℃ and it grew best between 6-8 pH value.Duojunling (carbendazim), Junxianwei (1.5% methane dithiocyanate), Lüxiang No.2 (80% carbendazim, thiram, ziram), etc.were effective for controlling the pathogenic bacteria in laboratory. Conclusion Isolation and identification of the pathogen can offer a theoretical foundation for the prevention of this disease.