乙肝病毒五项指标TRFIA定量测定与RIA检测结果的相关性研究

CORRELATIVE STUDY ON THE RESULTS OF TRFIA AND RIAIN DETECTING 5 HBV-M

目的 以微粒子酶免疫分析 (MEIA)为金标准 ,探讨时间分辨荧光免疫分析 (TRFIA)和放射免疫分析 (RIA)技术在定量检测乙肝表面抗原 (HBsAg)、表面抗体 (HBsAb)、e抗原 (HBeAg)、e抗体 (HBeAb)、核心抗体 (HBcAb) 5项乙型肝炎血清学标志物 (HBV -M )的临床应用价值。方法 应用MEIA、TRFIA及RIA 3种方法 ,对 2 0 0例乙肝病毒携带者血清进行HBsAg、HBsAb、HBeAg、HBeAb、HBcAb5项HBV -M指标检测。结果 TRFIA法检测HBsAg、HBsAb、HBeAg与MEIA结果完全符合 ,在HBeAb、HBcAb 2项指标中略高于MEIA法。TRFIA法及RIA法检测显示所选病例的HBsAg、HBsAb、HBeAg、HBeAb、HBcAb二者定量测定结果阳性符合率分别为96 .5 %、0 %、83.0 %、78.0 %、94 .2 %。与TRFIA法相比 ,RIA法检测HBsAg、HBeAg、HBeAb易出现漏检。TRFIA法检测HBsAg、HBsAb、HBeAg、HBeAb、HBcAb的诊断灵敏度、诊断特异性和结果总符合率均优于RIA法。结论 TRFIA法和RIA法在定量检测的HBV -M 5项指标结果具有相关性 ,但两种方法之间检测结果浓度绝对值不可比。TRFIA技术用于HBV -M检测 ,是一种灵敏的定量检测方法 ,具有特异性强、灵敏度高、线性范围宽、标准曲线稳定性好、无放射污染 ,易于自动化。

Objective To explore the clinical value of T RF IA and RIA in detecting 5 HBV-Ms in terms of HBsAg,HBsAb,HBeAg,HBeAb and HBcAb a ccording to the standard of MEIA.Methods MEIA,TREIA and RIA were used respectively to detect HBsAg,HBsAb,HBeAg,HBeAb and HBcAb in 200 cases of HB V.Results The contents of all HBV-Ms except for HBsAg were found lower through TREIA than through RIA.The results of detections of HBsAg,HBsAb an d HBeAg through TREIA coincided with those through MEIA,with other two being sli ghtly higher through TREIA.The coincidence rates of positive HBsAg,HBsAb,HBeAg,H BeAb and HBcAb according to HBsAg and RIA detection were 96.5%,%,83.6%,78.0 and 94.2% respectively.Missed detections might occure in RIA when contrasted with TR FIA.TRFIA functioned better than RIA in detection the 5 HBV-Ms for its high sens itivity,speficity and accuracy.There existed some correlation between the result s of RIA and TRFIA detections,but the abslute valaes of concentration did not co incfide with each other.Conclusion TRFIA detection of 5 HBV-Ms fu nctions well for its sensitivity,specificity,wide linear range,stable standard c urves,no radioactive pollution and convenient operation.