以GFP为报告基因的酿酒酵母mRNA在哺乳动物细胞中的表达

The expression of green fluorescent protein gene mRNA from yeast in mammalian cell

目的 研究酵母mRNA在哺乳动物细胞中的翻译表达。方法 半乳糖诱导GFP基因在酿酒酵母细胞中表达,提取其mRNA ,RT -PCR鉴定,并以脂质体为介导,对血管内皮细胞HUVEC以及绿猴肾细胞COS - 7进行体外转染。结果 被转染HUVEC和COS - 7细胞分别出现绿色荧光,报告基因GFPmRNA在动物细胞中被识别、翻译。结论 提示酵母mRNA可能作为基因治疗物质应用于口服基因治疗的研究。

Objective To study the possibility of translation and expression of mRNA from recombinant yeast in mammalian cells. Methods The expression of GFP gene in recombinant yeast was induced by galacose. The mRNA was extracted and identified by RT-PCR and was transfected with liposome into mammalian cell HUVEC and COS-7. Results Green fluorescence appeared in transfected cells and mRNA of GFP gene was translated and expressed in HUVEC and COS-7 cells. Conclusion mRNA of recombinant yeast may be used as novel gene therapy materials for oral gene therapy.