反相高效液相色谱法测定不同产地白花蛇舌草中的对香豆酸

Determination of p-Coumaric Acid in Hedyotis diffusa Willd. from Different Sources by Reversed-Phase High Performance Liquid Chromatography

用反相高效液相色谱法对不同产地的白花蛇舌草中对香豆酸的含量进行了测定。色谱柱采用DiamonsilTMODS柱(4 6mmi d ×250mm, 5μm),流动相为乙腈 20mmol/LNH4Ac(pH4 0) (体积比为 15∶85),流速 1 0mL/min,柱温为室温,检测波长为 308nm。在上述条件下测得对香豆酸的质量浓度为 4 04~202mg/L时与色谱峰面积之间线性关系良好(r=0 999 6),高中低浓度条件下的平均回收率为 97 4%~102 2%。该方法快速简便,精密度高,重现性和准确度良好,可以作为白花蛇舌草质量控制的一个有效方法。

A method for the determination of p-coumaric acid in Hedyotis diffusa Willd. from different sources by reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. p-Coumaric acid was successfully separated on a Diamonsil^(TM) ODS column (4.6 mm i.d.×250 mm, 5 μm) at ambient temperature, using a mixture of CH_3CN-20 (mmol/L) NH_4Ac (pH 4.0) (15∶85, v/v) as mobile phase and detection at 308 nm. The flow rate was 1.0 (mL/min). There was good linear relationship (r=(0.9996)) between the mass concentration and the peak area of p-coumaric acid in the range of 4.04-202 mg/L. The recoveries were found to be in the range of 97.4%-102.2%. The results of the experiments have demonstrated that the established method is rapid and simple with good accuracy and reproducibility. The method is suitable for use in quality control of Hedyotis diffusa Willd. from different sources.