黑曲霉产α-转移葡萄糖苷酶固态发酵条件优化

Studies on Solid-State Fermentation Conditions for Producing α-Transglucosidase from Asp.niger

对产α 转移葡萄糖苷酶的黑曲霉 U菌株固态发酵条件进行了优化.结果表明:麸皮和水的质量比为1∶1较好,35 ℃培养48 h时产酶量及酶活较高; U菌株生长的最适pH值为6 0; 麸皮中的碳源和氮源可以满足该菌株产酶的需要,可以不外加碳源和氮源.

Alpha-transglucosidase is a critical enzyme for producing isomaltooligosaccharide. The solid-state fermentation conditions for producing α-transglucosidase from Asp.niger U were studied. The optimum conditions for fermentation in solid-state were:bran:water(m/m) =1∶1,culture temperature and duration were 35 ℃ and 48 h respectively, the optimum pH value was (6.0). Addition of carbon and nitrogen sources into the culture medium of bran was not required, as the carbon and nitrogen sources in bran could satisfy the needs for the enzyme production.