培养基和植物激素对球根秋海棠微体快繁的影响

Effect of Culture Media and Hormones on Breeding of Begonia tuberhybrida Leaf

探讨了培养基、大量元素和植物激素对球根秋海棠微体快繁的影响。结果表明 :在诱导外植体分化形成愈伤组织的过程中以BA、NAA较为适宜 ;增殖培养时以MS + 6 BA(0 .8～ 1.2 )mg/L +NAA 0 .1mg/L +糖 3 0g/L +琼脂 7g/L为培养基 ,有利于提高球根秋海棠的分生倍数 ;而生根时以 1/2MS +IAA 0 .5mg/L +糖 2 0g/L +琼脂 7g/L为培养基 ,则有利于提高生根率。

The effect of macro-element and phytohormones on quick reproduction of Begonia tuberhybrida leaf was studied. The results showed culture medium of MS+ 6-BA(0.8～1.2)mg/L+NAA 0.1mg/L+Suger 30g/L+Agar 7g/L was suited for the regeneration of Begonia tuberhybrida leaf for the flower industry or reproduction process.Culture medium of 1/2MS+IBA 0.5mg/L+Suger 20g/L+Agar 7g/L was suitable for shoot after root was regenerated.