天芎注射液中多糖的含量测定

Determination of polysaccharide in Tianxiong injection by colorimetry

目的 建立天芎注射液中多糖含量的测定方法。方法 硫酸蒽酮作为显色液,采用比色法测定天芎注射液中多糖的含量。结果 线性范围为10～5 0 μg·ml-1(r=0 . 9999) ;高、中、低浓度平均回收率为98. 7% ,RSD =0 .83% (n =6 )。结论 所用方法操作简便,重复性好,适用于天芎注射液中多糖的含量测定。

OBJECTIVE To establish a method for the determination of polysaccharide in Tianxiong injection. METHODS Polysaccharide in Tianxiong injection was determined by colorimetry. RESULTS The linear range of polysaccharide was 10-50 μg·ml -1 (r=0.9999);The average recovery of this method was 98.7% with RSD of 0.83%(n=6). CONCLUSION The method is simple, accurate , reproducible and suitable for the determination of polysaccharide in Tianxiong injection.