HBsAg阴性的慢性HC病人血清中HBV-DNA检测

HEPATITIS B VIRUS DNA IN SERA OF HBSAG NEGATIVE PATIENTS WITH CHRONIC HEPATITIS C.

目的:检测慢性HC病人血清中HBV—DNA阳性率。方法:用巢式PCR对3 8例HBsAg阴性的HC病人作HBV—DNA检测,选出3 0例慢性HB病人血清作对照组。结果:测定组HBV—DNA阳性为4 5 % ( 1 7/ 3 8) ,血清转氨酶值、肝炎活动度、肝纤维化程度、HBV感染标志物等指标在2种HC病人(HBV—DNA阴性和阳性)中差异均无显著性(P >0 .0 5 )。结论:用PCR可以在一部分HBsAg阴性的慢性HC病人血清中检测到HBV—DNA。HBV可能抑制HCV复制。用PCR多次重复测定HBV—DNA可以作为排除HCV、HBV混合感染的方法。

Objective:To determine the rate and explore the clinical significance of HBsAg negative HBV patients coinfectied with chronic hepatitis C.Methods:Sera from 38 HBsAg negative patients with chronic hepatits C were examined for HBV DNA by nested polymerase chain reaction (PCR).For control purposes,Sera from 30 patients with chronic HBV infection (8 HBsAg positive,7 HBsAg negtive,all HBV PCR positve)were examined.Results:HBV DNA was detected in 45% of patients with chronic hepatitis C.There was no significant difference in mean aminotransferease values,inflammatory diease activity and degree of hepatic fibrosis observed in patients with or without HBV DNA.Negative HCV PCR was more common among patients with positive HBV PCR in serum.Conclusion:Coinfection with HBV can be demonstrated by PCR in a considerable number of HBsAg negative patients with chronic hepatitis C.HBV infection seems to suppress HCV replication even in HBsAg negative patients with dual infection .HBV coinfection in HCV infectioned patients can not be excluded by negative HBsAg status alone.