温度对采后梨果实多胺、ACC含量、EFE活性和乙烯生成量的影响

Effect of Temperature on Polyamine, ACC Contents, EFE Activities and Ethylene Production in Post-harvested Pear Fruits

测定了两个日本梨品种菊水和二十世纪(Pyrus serotina Rehder var.culta.Rehder)在30、15和5℃贮藏温度下,多胺、ACC含量、EFE活性和乙烯释放速率的变化。1.15和5℃低温可显著抑制梨果实内EFE活性,乙烯生成速率显著降低;2.两品种对低温的敏感性存在差异,15℃低温处理既能减少二十世纪梨的乙烯生成速率,也能抑制乙烯释放高峰的出现,而同样的温度处理仅能减少菊水梨的乙烯生成速率,不能抑制乙烯释放高峰的出现;3.在15和5℃处理下,两品种果实内ACC含量和丁二胺含量均呈上升趋势,5℃处理比15℃处理明显;4.梨果实内含有3种多胺,其中以丁二胺和亚精胺为主,丁二胺与梨果实内乙烯生物合成的抑制作用有密切关系;5.品种间在EFE活性、ACC含量和多胺含量上的差异决定了它们在乙烯生成速率上的差异。

Kikusui pears and Nijisseiki pears ( Pyrus serotina Rehder var. culta. Rehder) har-vested at 140 days after full bloom were kept at 30, 15 and 5℃ . The activities of ethylene forming enzyme (EFE) and the rate of ethylene production were enhanced at 30℃ while the reverse was true at 15 and 5℃. The apperance of ethylene peak was also delayed or in-hibited at 15 and 5℃. Effect of temperature on ethylene production varied in different vari-eties. It was found that the fruits kept at 15 and 5℃ had higher polyamine and ACC con-tents than those at 30℃. A mechanism that the rate of ethylene production was influenced by polyamine and ACC contents under different temperatures was postulated in this article. It seemed that putrescine played a more important role in restraining ethylene biosynthesis of pear fruits than spermidine and spermine.