多效唑在唐菖蒲组织培养中的作用

The Effect of Paclobutrazol on Gladiolus in Tissue Culture

在ＭＳ＋蔗糖６０ｇ／ｋｇ的固体培养基中加入不同浓度的多效唑（ＰＰ３３３），对组培唐菖蒲球茎形成有不同程度的促进作用，其中浓度为３０ｍｇ／ｋｇ的球茎形成较早；浓度为３和１０ｍｇ／ｋｇ的形成球茎较大。在多效唑１０ｍｇ／ｋｇ的培养基上，接种芽的长度不同对球茎形成也有影响，３～５ｃｍ的芽球茎形成较早；太长的芽，球茎形成反而推迟。活性炭对多效唑有明显吸附作用，不仅球茎形成推迟，球茎鲜重也下降。

Paclobutrazol at different concentrations improved gladiolus corm formation obviously when the shoots were cultured on the solid agar mediums which did not contain any other regulators (MS+sucrose 60g/kg). Corms formed earlier on the medium which contained 30mg/kg paclobutrazol, and corms formed larger when paclobutrazol was at 3～10mg/kg. Different length of shoots had some effect on corm formation when the shoots were cultured on the same medium which contained 10 mg/kg paclobutrazol. The inoculated shoots at 3～5cm formed earlier and larger, too long shoots delayed corm formation relatively. Active carbon had absorption effect on paclobutrazol obviously. It delayed corm formation and reduced fresh weight of corm.