心衰患者T细胞CD25水平及卡维地洛的体外影响

Expression of CD25 on T lymphocyte surface in congestive heart failure and the effect of carvedilol on its expression in vitro

目的:观察慢性心衰患者外周血T细胞CD25表达水平,探讨卡维地洛(Car)体外对外周血T细胞CD25表达的作用。方法:分离外周血单个核细胞,用卟啉醇肉豆蔻酸乙脂(PMA)+A23187作为刺激剂,加或不加Car,分组体外培养。以流式细胞仪检测T淋巴细胞活化抗原CD25/CD3。结果:(1)与正常人相比,慢性心衰患者T细胞CD25 表达水平明显升高(P<0.001);(2)与对照组相比,10μMCar可以抑制T淋巴细胞CD25抗原的表达(P<0.05)。结论:慢性心衰患者外周血T细胞CD25表达水平明显升高;Car体外可以抑制其表达。

Objective:To observe the expression of CD25 on T lymphocyte surface in congestive heart failure (CHF) and the effect of carvedilol on its expression in vitro. Methods:Peripheral blood mononuclear cells (PBMC) were prepared from normal donors, and incubation with phorbol 12-myristole 13-acetate (PMA) and A23187 with or without carvedilol in vitro. After incubation, the expression of CD25 and CD3 on cell surface was assayed with flow cy-tometry. Results:(1)The expression of CD25 on T lymphocytes surface in CHF was significantly higher than that of normal (P<0. 001);(2) Carvedilol (10μM) significantly inhibited the expression of CD25 on T lymphocyte surface (P<0.05). Conclusion:The expression of CD25 on T lymphocytes surface in CHF significantly increase, the carvedilol can inhibit its expression in vitro.