青花菜花芽分化前后内源激素含量及酶活性的变化

The changes of endogenous hormones and enzymes activity during the flower-bud differentiation in broccoli(Brassica oleracea var Italica)

青花菜的花芽分化过程伴随着GA3、IAA的含量、POD和转化酶的活性变化。当植株进入花芽分化临界期时,叶片中GA3含量以及POD和转化酶活性均开始剧增,而IAA含量开始剧减;当植株进入第1侧花茎原基分化期和第2～3侧花茎原基分化期,叶片中GA3含量和POD、转化酶活性均出现一高峰值,而IAA含量出现一低峰值。可见,青花菜侧花茎每一级的花芽分化要求较高含量的GA3、较低含量的IAA和较高活性的POD、转化酶。

The flower-bud differentiation was accompanied by the changes of GA3 contents, IAA contents, POD activities and invertase activities in broccoli. GA3 contents, POD activities and invertase activities started to increase significantly while IAA contents started to decrease significantly when plants entered the critical stage of flower-bud differentiation; GA3 contents, POD activities and invertase activities emerged high apex value while IAA contents emerged low apex value when plants entered stage of primary furcation scape primordium differentiation and stage of secondary and tertiary furcation scape primordium differentiation. In a word, higher GA3 contents, lower IAA contents, higher POD activities and invertase activities were required at each grade of furcation scape primordium differentiation stage of broccoli plant.