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siRNA对鸡胚成纤维细胞中GAPDH基因表达的抑制作用 被引量：4

siRNA inhibit GAPDH gene expression in chicken embryonic fibroblast cells
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摘要 RNA干扰(RNA interference,RNAi)是通过短双链RNA(small interference RNA,siRNA)介导的特异性抑制相应基因表达的新技术。作为研究基因功能的有效手段,该技术已在线虫、果蝇、斑马鱼和鼠等动物中进行了应用。本研究利用体外转录合成的短双链干扰RNA(siRNA),针对鸡的管家基因3-磷酸甘油醛脱氢酶基因(glyceraldehyde-3-phosphate dehydrogenase,GAPDH),在鸡胚成纤维细胞中对其表达进行干扰。结果显示siGAPDH能有效阻断GAPDH基因在鸡胚成纤维细胞中的表达,而不相关的siRNA则对该基因的表达没有影响,证明在鸡细胞中也存在RNA干扰现象,为利用RNAi技术对鸡的基因功能研究奠定了一定基础。 RNA interference (RNAi) is a new methodology to inhibit gene expression specifically by small interference RNA triggering. As an efficient approach, it has been widely used to study gene function on Caenorhabditis elegans. Drosophila, zebrafish and mouse. Here, we inhibit the expression of GAPDH gene in chicken embryonic fibroblast cells by used siRNA that was synthesized through transcription in vitro. The result shows that the siRNA can inhibit the expression of GAPDH gene efficiently but the siIRR can not. Its means there is RNAi mechanism in chicken embryonic fibroblast cells. This experiment will be helpful to the researching gene function of chicken by used RNA interference.
作者 陈学辉 孟和 潘玉春 崔芳岩
机构地区 东北农业大学动物科学技术学院 上海交通大学农业与生物学院
出处 《东北农业大学学报》 CAS CSCD 2005年第2期199-203,共5页 Journal of Northeast Agricultural University
基金 国家"973"计划资助项目(2003CB514129)
关键词 RNAI 鸡胚成纤维细胞:GAPDH RNAi chicken embryonic fibroblast cell GAPDH
分类号 S831 [农业科学—畜牧学] Q343.1 [生物学—遗传学]
  • 相关文献

参考文献30

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共引文献31

同被引文献53

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引证文献4

二级引证文献16

东北农业大学学报
2005年 第2期

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