牛膝中三萜皂苷类化合物的电喷雾电离串级质谱研究

Studies on Triterpene Saponin from Achyranthes bientata BI by Electrospray Ionization Tandem Mass Spectrometry

采用大孔树脂柱层析分离方法分离提取牛膝 (Achyranthesbientata BI)中的三帖皂苷类化合物。通过相对分子质量及电喷雾电离多级串连质谱 (ESI-MSn)技术分别对牛膝提取物中的有效成分牛膝皂苷 (相对分子质量 95 6)、牛膝皂苷 (相对分子质量 794)进行定性分析 ,并提出了其电喷雾质谱碎裂机理。该方法样品处理简单 ,分析速度快且灵敏度高 ,适用作为牛膝有效成分三萜皂苷类物质鉴定的依据。

AchyranthosideI and Achyranthoside Ⅱ which were considered to be effective constituents from Achyranthes bientata BI were identified by electrospray ionization tandem mass spectrometric (ESI-MSn) methods after macro-porous adsorbent chromatographic separation.Their molecule masses were detected and ESI-MSn date were obtained, and fragmentation mechanisms were proposed. Their characteristic mass spectra can be use as fingerprint for the identification of AchyranthosideI and Achyranthoside Ⅱ. This method is simple, rapid and highly sensitive.