中国野生葡萄葛蘲叶绿体DNA质粒文库的构建

CONSTRUCTURE OF THE PLASMID LIBRARY OF cpDNA FROM VITIS FLEXUOSA IN CHINA

限制性内切酶ＢａｍＨＩ水解中国野生葡萄葛（ＶｉｔｉｓｆｌｅｘｕｏｓａＴｈｕｎｂ）的叶绿体ＤＮＡ（ｃｐＤＮＡ），电泳分离为３４条带，最大为１１．７ｋｂ，最小为０．２３ｋｂ，分别用ｐＵＣ和ｐＢＲ３２２质粒克隆所得片段，转化大肠杆菌，并从转化菌中提取质粒ＤＮＡ，经酶切电泳分析证明含有葛ｃｐＤＮＡ经ＢａｍＨＩ水解的不同片段，从而构建了葛ｃｐＤＮＡ的ＢａｍＨＩ质粒文库。

Choroplast DNA from Vitis flexuosa Thunb was digested with BamHI.The resulting fraginents were cloned into plasmids pUC7,pUC9 and pBR322. The plasmid library of the BamHI fragments of cpDNA from Vitis flexuosa Thunb was obtained.