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根癌农杆菌对苜蓿的转化及其特性 被引量:4

The Agrobacterium tumefaciens-mediated transformation and characteristics of Medicago sativa
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摘要 通过胭脂碱型根癌农杆菌C58和T37菌株通过共培养和离体感染苜蓿下胚轴和子叶,研究表明,外 植体的生理状态对转化的促进作用明显优于酚类化合物As和复合酚类化合物处理。与感受态外植体相比, 当外植体处于非感受态时,即使通过酚类化合物活化农杆菌vir基因,其致瘤率也明显降低。可溶性蛋白质 含量和游离氨基酸含分析表明,T37和C58转化细胞系可溶性蛋白含量相近,但T37转化细胞系游离氨基酸 含量比C58转化细胞系高得多,这与T37和C58转化细胞系生长特性有关。T37菌株的这种特性对于改良 苜蓿品质具有非常重要的现实意义。 By in vitro infecting hypocotyls and cotyledons with nopaline-type Agrobacterium tumefaciens strain C58 and T37,we concluded that the promotional role of physical status of explants was obviously prior to that of acetosyringone and complex phenolic compounds. Compared to competent explants,non-competent explants had an obvious lowered tumorigenic frequency,even if acetosyringone and complex phenolic compounds were treated to activate vir gene of A.tumefaciens. The analysis of soluble protein contents and free amino acid contents showed that T37 and C58 transformed cell line had similar soluble protein contents,but T37 transformed cell line had much higher free amino acid contents than C58 transformed cell line and this was correlated to their growth characteristics. The characteristic of T37 strain might be used to improve the quality of Medicago sativa.
作者 刘明志
出处 《广西植物》 CAS CSCD 北大核心 2005年第2期121-124,i005,共5页 Guihaia
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参考文献11

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