宫内感染乙型肝炎病毒儿童白细胞介素12转录研究

Transcription of the messenger RNA of interleukin 12 in intrauterus infected hepatitis B children

目的 探讨外周血单个核细胞白细胞介素 12 (IL-12 )P40转录变化和宫内感染乙型肝炎病毒 (HBV)免疫失败的关系。方法 采用体外细胞培养和半定量RT PCR技术对 2 5例宫内感染免疫失败儿童、8例宫内感染有效儿童及19名正常免疫儿童外周血单个核细胞在丝裂原 (植物血凝素和细菌酯多糖 )、酵母重组乙型肝炎表面抗原 (HBsAg)及无刺激物时IL-12P40mRNA转录水平进行检测。结果 IL 12P40mRNA丝裂原刺激时转录在免疫失败组高于免疫有效儿童 (P =0 .0 42 ) ,自发转录和血清丙氨酸转氨酶水平呈显著正相关 (r =0 .3 89,P =0 .0 0 4)。在小剂量HBsAg刺激时 ,对照组、有效组和免疫失败组转录差异不明显。和小剂量HBsAg刺激时比较 ,大剂量HBsAg刺激时对照组表现为转录显著增加 (P =0 .0 3 9) ,有效组和免疫失败组变化不明显。结论 宫内感染HBV儿童对特异性抗原刺激IL-12反应低下可能是导致免疫失败的机制之一 。

Objective To investigate the mRNA response of interleukin 12 (IL12) in immunization failure children who were infected hepatitis B virus (HBV) via placenta from HBV carrier mothers.Methods All subjects were born to HBV carrier mothers and reveived hepatitis B immunoprophylaxis according to routine schedule. IL 12 P40 mRNA transcription of peripheral blood mononuclear cells upon lectins or two-doses of HBsAg stimulation from 25 children with persistent HBV infection since birth(Nonresponders), 8 with transient HBV infection at birth and at less than 6 month of age (Responders) were analyzed by semi-quantitative RT-PCR. 19 children with positive anti-HBs response and never positive HBsAg were used as controls.Results Nonresponders expressed a higher IL12 P40 mRNA transcription than responders upon lectins stimulation (P=0.042). Positive conrrelations were found between ALT level and the IL 12 P40 mRNA transcription without stimulators((r=0.389),(P=0.004)). There were no significant differences of IL 12 P40 mRNA transcription between the three groups upon low dose of HBsAg stimulation. Upon high dose of HBsAg stimulation, control group expressed a significant higher IL12 P40 mRNA transcription than low dose of HBsAg stimulation(P=(0.039)),but no significant differences were found in nonresponders and responders.Conclusion Low responses of IL 12 P40 mRNA transcription upon specific stimulation exist in intrauteral infected children, and the increase of response of it upon nonspecific stimulation may result from the liver injury in nonresponders.