小剂量吉西他滨可促进放疗致人肝癌细胞株SMMC-7721的凋亡

Low-dose gemcitabine promotes radiation-induced apoptosis of human hepatoma cell line SMMC-7721

目的观察小剂量吉西他滨(GEM)对放疗诱导的人肝癌细胞株SMMC-7721凋亡的促进作用。方法将体外培养的人肝癌细胞株SMMC-7721分为空白对照、单纯放疗、单纯化疗及放疗加化疗4组,应用MTT法检测不同条件下SMMCC-7721细胞的生长活性;经光学显微镜、透射电镜及荧光显微镜观察各组细胞经GEM作用后凋亡的形态学变化。结果单纯放、化疗都可诱导GEM细胞凋亡,GEM可明显增加放疗诱导的细胞凋亡。结论小剂量GEM可明显增加放疗诱导的SMMC-7721细胞的凋亡。

Objective To observe the effect and explore the mechanism of low-dose gemcitabine (GEM) in promoting the apoptosis of human hepatoma cell line SMMC-7721 induced by radiation. Methods The SMMC-7721 cells were divided into four groups to serve as the control group or exposed to treatment with radiation only, GEM only, and both GEM and radiation, respectively. MTT assay was used to evaluate the activity of SMMC-7721 cells at different conditions, and the apoptosis rate and morphologic changes of the cells after treatment were observed under optical microscope, transmission electron microscope and fluorescence microsope, respectively. Results Treatment with either radiation or GEM alone was capable of inducing cell apoptosis, and GEM significantly increased the cell apoptosis induced by radiotherapy. Conclusion Low-dose GEM can significantly enhance radiation-induced apoptosis of SMMC-7721 cells.