兜唇石斛多糖的研究

STUDIES ON THE POLYSACCHARIDES OF DENDROBIUM APHYLLUM

从兜唇石斛（Ｄｅｎｄｒｏｂｉｕｍａｐｈｙｌｌｕｍ）的茎中提取到３种多糖：ＡＰ－１，Ａｐ一２，Ａｐ一３，玻璃纤维纸电泳、超速离心和凝胶层析证明：三者都为单一均匀成分。３种多糖的特性粘数（［ｎ］）分别为４２６．９６，４１６．９６，２３４．０３；分子量（Ｍｗ）分别为８６３００，６１５００，４３１００；旋光度（）分别为－３０．０００，－３３．０００，１７．０００。经化学方法和光谱分析证明：ＡＰ为Ｂ（１→４）连接的、含Ｏ一乙酰基的、吡喃型的直链Ｄ一葡萄甘露聚糖。动物体内试验表明：ＡＰ有免疫增强作用。

Three polysaccharides,namely AP-1,AP-2,AP-3,which were proved to be homo-geneous by glass fiber paper electrophoresis,ultracentrifugation analysis and gel chromatography,were isolated from the stems of Dendrobium aphyllum The limiting viscosity numbers of three polysaccharides were 426.96, 416.96 and 234.03 respectively.Their molecular weights(Mw)were 86300, 61500 and 43100 respectively.The numbers of their optical rotation were-30.00,-33.000 and-17.000 respectively.It was proved by chemical methods and spectrum analvsis that:APs were O一acetylglucomannan,the linkage was B(1→4), the ring foml of each glycosyl residue was pyranose and the main chain was composed of D一mannose and D一glucose. The animal experimentation in vivo showed AP had evident immunostimulating activity.