三羟异黄酮对人乳腺癌细胞株MCF-7细胞增殖和细胞周期的影响

The effects of genistein on the proliferation and cell cycle in human breast cancer cell MCF-7

目的:研究三羟异黄酮(genistein,Gen)对乳腺癌细胞株MCF鄄7细胞增殖和细胞周期的影响。方法:采用MTT比色法观察Gen对MCF鄄7细胞增殖的影响,流式细胞仪分析细胞周期分布,Westernblot分别检测周期素B(cyclinB)、周期素E(cyclinE)蛋白表达情况。结果:Gen(≥10μmol/L)对MCF鄄7细胞生长有显著抑制作用,细胞生长阻滞于G2/M期,cyclinB蛋白表达增加,且呈剂量-效应关系;但对cyclinE蛋白表达无影响。结论:Gen抑制MCF鄄7细胞增殖、诱导G2/M期阻滞与其导致cyclinB蛋白积累有关,与cyclinE蛋白表达无明显关系。

Objective:To study the effects of genistein(Gen) on the proliferation and cell cycle progression in human breast cancer cell MCF-7. Methods:The effects of Gen on the growth of MCF-7 cells were observed by MTT assay. Flow cytometry was used to analyze the cell cycle. The cyclin B and cyclin E protein expressions were observed using Western blotting method. Results:Gen inhibited the proliferation of MCF-7 cells significantly, arrested cell cycle progression at G2/M phase, and enhanced cyclin B protein expression with dose-dependent manner. No effect on cyclin E protein expression was observed. Conclusion:Growth inhibition and G2/M arrest of MCF-7 cells after treated with Gen may be related to the accumulation of cyclin B protein,rather than cyclin E protein expression.