茶多酚对高糖时内皮细胞产生活性氧和TGF-β_1的影响

The effect of tea polyphenols on the production of ROS and TGF-β_1 by endothelial cells in high glucose culture media

目的探讨高糖对内皮细胞产生活性氧和转化生长因子β1(TGF β1)的影响,以及茶多酚的干预作用。方法培养人脐静脉血管内皮细胞(ECV304),分为正常对照组、高糖组、茶多酚组和茶多酚干预组。培养0、12、36 h后,用分光光度比色法测定细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,用RT PCR法测定细胞内TGF β1 mRNA的变化。结果高糖导致内皮细胞SOD活性下降和MDA含量升高,上调TGF β1 mRNA表达,其变化随时间延长更为明显。茶多酚干预可拮抗高糖时内皮细胞的上述改变。结论茶多酚有抑制高糖对内皮细胞的损伤作用。

Objective To investigate the effect of high glucose on the production of reactive ~oxygen species (ROS) and transforming growth factor β_1 (TGF-β_1) in human umbilical vein endothelial cells(ECV304),and the intervention of tea polyphenols (TPs). Methods ECV304 was cultured ~in different conditional media and divided into control group, high glucose group, TPs group and TPs treatment group. After 0,12 and 36h, the activity of SOD and the contents of MDA in supernatant were detected by spectrophotometry, meanwhile the mRNA expression of TGF-β_1 mRNA was determined by RT-PCR. Results The activity of SOD was found to be significantly decreased , while the contents of MDA and the expression of TGF-β_1 mRNA increased in high glucose. Such changes ~became more significant as time prolonged.TPs could inhibit all these changes of ECV304 in high ~glucose. Conclusion TPs may diminish the harmful effect of high glucose on the endothelial cells.