茶树叶糖蛋白分离及鉴定的初步研究

Study on isolation and determination of glycoprotein from tea plant leaves

茶叶是一种有益健康的保健食品,保健功能在于它有多种化学活性成分.近年来研究表明,茶叶糖蛋白可能是茶叶的保健因子之一.从茶树叶中提取了总蛋白、45%～70%硫酸铵分级总蛋白、分级蛋白Sephadex G-100凝胶层析分离出粗糖蛋白,根据糖蛋白糖链及蛋白的对照染色鉴定出多种茶树叶糖蛋白,并从SDS-胶上快速纯化了三种茶叶糖蛋白.ConA-Sepharose 4B亲和层析从茶树叶总蛋白中分离出16种天然活性糖蛋白.

Tea is a healthy food, beacause it has various bioactive components. Recent research indicated that tea glycoprotein might be a bioactive component in tea. In this report, total proteins were extracted from tea fresh leaves, and coarse glycoproteins were isolated through the methods of ammonium sulfate fractions (45%~70%) and SephadexG-100 chromatography. According to control staining of glycan and polypeptide, glycoproteins were determinated by using Pierce GelCode Glycoprotein Staining Kit and Coomassie Brilliant Blue R250, and three tea glycoproteins were fast purified from the SDS-gels. Sixteen native bioactive glycoproteins were gained from tea total proteins by ConA-Sepharose 4B affinity chromatography.