灵芝孢子几种破壁方法比较分析

Comparing analysis on several wall-cracking methods of Ganoderma lucidum spore

采用超临界破壁法、匀浆破壁法和超声破壁法对灵芝孢子进行了破壁试验研究,研究显示,采用超临界高压静止破壁法可显著提高灵芝孢子的破壁率。匀浆破壁法和超声破壁法虽灵芝孢子破碎率较低,显微镜观察大部分孢子外形完整,但细胞内容物已几乎消失,达到提取孢子内容物的目的。

Several methods on the wall-cracking of Ganoderma lucidum spore were carried on, mainly wall-cracking methods included supercritical wall-breaking, homogenate wall-cracking and ultrasonic wall-breaking. The results showed that adopting supercritical high-pressure steady state wall-cracking method could particularly improve the wall-crushing rate of Ganoderma lucidum spore. Although the wall-cracking rate of homogenate wall-cracking and ultrasonic wall-cracking was lower and most of the spores of Ganoderma lucidum were integrate in the microscope,the contents were nearly disappeared, attach to the purpose extracting effective constituentof spore inner.