Cx43基因在人类及小鼠胎心发育中的时空表达规律

Spatio-temporal Expression of CX43 in the Developing Human and Mouse Hearts

目的 检测Cx4 3在人类和小鼠的胚胎心脏的表达 ,了解该基因在心脏发育过程中的表达规律。方法 选取人类 6～ 18孕周正常胚胎或胎儿心脏 6 3例 ,小鼠孕龄 9 5～ 16 5d胚胎心脏 6 4例 ,采用免疫组化法显示Cx4 3基因在心脏的表达。结果 早期人类胚胎心脏中 ,Cx4 3在心室肌中没有表达 ,心房肌表达微弱 ,原始小梁网中表达很高 ,随着胚胎发育 ,在心房和心室的表达逐渐增强 ,小梁网的表达在胚胎 13～ 14周达到高峰。室间隔的肌部表达量较弱 ,膜部室间隔不表达。房室瓣和大动脉根部管壁Cx4 3没有明显表达。除了在大动脉管壁表达不同 ,小鼠胚胎心脏表达规律与人类基本相同。结论 Cx4 3对于胚胎心脏的发育至关重要。

Objective To investigate the spatio-temporal expression of CX43 i n the developing human and mouse hearts. Methods 63 normal human fetal hearts i n 6-18 gestational weeks and 64 normal mouse fetal hearts in embryonic days 9.5 -16.5 were included in this study. Immunohistochemistry was used to detect th e CX43 expression. Results In early stage of development of hu man fetal heart, no CX43 expression was detected in ventricular myocardium and w eak expression in atrial myocardium. High expression was observed in primitive t rabecular nets. With the fetal development the CX43 expression was enhanced in t he atria and ventricles. Peak expression was revealed in trabecular net at 13 - 14 weeks. Expression was weak in muscular interventricular septum. No expressio n was observed in membranous interventricular septum, atrial-ventricular valves and large arteries. Except for the large arteries, CX43 expression in the devel oping mouse heart was similar to that in human fetal hearts. Conclu- s ion CX43 may play a crucial role in the heart development.