Cobe Spectra与Fenwal CS 3000 Plus血细胞分离器外周血造血干/祖细胞的采集效能比较

Comparison of the Effect of Cobe Spectra and Fenwal CS 3000 Plus Blood Cell Separators in Collection of Peripheral Blood Stem Cell Components

为了评价Cobe Spectra(Version 6.1)和Fenwal CS 3000 Plus两种血细胞分离机在造血干／祖细胞采集中的 采集效能,对36人64次外周血造血干／祖细胞的采集进行了回顾性分析。20人42次使用Cobe Spectra采集,16人 22次使用Fenwal CS 3000 Plus采集。对CD34+细胞采集量、采集效率以及红细胞与血小板的采集量进行比较。结 果表明:Cobe Spectra和Fenwal CS 3000 Plus在CD34+细胞采集量和采集效率上无显著差异。CD34+细胞采集量 与供者白细胞、单核细胞、造血祖细胞、CD34+细胞的动员情况呈正相关,在多因素stepwise线性回归模型中,采集 前外周血干／祖细胞浓度是唯一有意义的影响因素。Fenwal CS 3000 Plus的采集效率与外周血单核细胞量呈负相 关。Fenwal CS 3000 Plus对红细胞的收集量显著高于Cobe Spectra,并且采集后供者外周血血小板降低程度较Cobe Spectra更严重。结论:Cobe Spectra(Version 6.1)和Fenwal CS 3000 Plus在CD34+细胞的采集能力上无显著差别。 当外周血单核细胞数量高,或是血型不合移植的及血小板减少的供者,推荐使用Cobe Spectra血细胞分离机。

To evaluate the hematopoietic stem/progenitor cell apheresis effect of Cobe Spectra (Version 6. 1) and Fenwal CS 3000 Plus cell separators, fourty-two procedures on twenty donors using Cobe Spectra cell separator and twenty-two procedures on sixteen donors using Fenwal CS 3000 Plus cell separator were retrospectively analyzed. The number of CD34+ cells collected, the collection efficiency (CE) of CD34+ cells and the contaminations of red blood cell and platelet in the stem/progenitor cell products of two devices were compared. The results showed that there were no significant differences in the total number of CD34+ cells collected and the CD34 + cell CE between the two devices. There were positive correlations between the count of peripheral blood cells including leukocyte, monocyte, hematopoietic progenitor cell and CD34 + cell after mobilization and the total number of CD34 + cells collected. The stepwise multiple variable analyses revealed the peripheral blood stem/ progenitor cell count emerged as the only significant independent predictive factor for CE. A negative correlation was seen between the peripheral blood monocyte count and the CD34 + cell CE for the Fenwal CS 3000 Plus. The Fenwal CS 3000 Plus product contained more red blood cells than that of the Cobe Spectra. The decrease in the peripheral platelet count after Fenwal CS 3000 Plus apheresis was also greater. It is concluded that collection efficacy of Cobe Spectra (Version 6.1) and Fenwal CS 3000 Plus was similar. Cobe Spectra shall be used preferably to assure higher CD34+ cell CE at a high peripheral blood monocyte count. The Cobe Spectra cell separator is better for the donors with mismatched blood type and the donors with thrombocytopenia.