115例急性髓细胞白血病多参数流式细胞术免疫表型分析

Immunophenotypes in 115 Patients with Acute Myeloid Leukemia by Multi-color Flow Cytometry

免疫表型检测已成为白血病诊断和分类的重要手段。为了解急性髓细胞白血病(AML)免疫表型特征,利用 三色流式细胞术CD45／SSC参数散点图设门方法,对115例AML患者幼稚细胞表面及胞浆内分化抗原进行分析;结 合FAB分类,比较AML不同亚型中抗原表达的差异,并对其诊断价值加以探讨。结果显示:在AML患者中,CD33、 CD38和CD13的表达最常见,分别达94.8％、91.3％、89.6％。在淋系抗原中,CD7的表达最为常见(20.2％),其次是 CD19(16.5%)和CD2(15％)。某些免疫表型特征与FAB分类具有相关性,包括M3中缺乏表达HLA-DR、CD34和 CD56,但CD2的表达增加;M2中CD19,M5中CD14和CD56的表达增加,而M0中未见MPO的表达。结论:多参数 流式细胞术是诊断AML的一项可靠技术,AML某些免疫表型特征与FAB分类具有明显相关性。

Immunophenotyping has become common in the diagnosis and classification of leukemia. To evaluate the immunophenotype of acute myeloid leukemia (AML), multiparameter flow cytometry and CD45/SSC gating were used to analyze the surface and cytoplasmic antigen expressions in 115 cases of AML. The results were compared with the French-American-British (FAB) Cooperative Group classification to help define the best use and role of multiparameter flow cytometry in the diagnosis and proper classification of AML. The results showed that CD38, CD38 and CD13 were the most commonly expressed antigen (94. 8% , 91. 3% and 89. 6% , respectively). CD7 was the most commonly expressed lymphoid antigen (20. 2% ), followed by CD19 ( 16. 5% ) and CD2 ( 15% ). Some immunophenotypes correlated with FAB type, including increased frequency of CD2 in M3; lack of HLA-DR, CD34 and CD56 expression in M3; increased frequency of CD19 in M2, CD14 and CD56 in M5 and lack of MPO in M0. In conclusion, multiparameter flow cytometry is a reliable technique in the diagnosis of AML, and some immunophenotypes correlate with FAB type.