系统性红斑狼疮患者T细胞上Fas受体分子与疾病活动指数的相关性研究

The correlation between quantitative expression of Fas receptor molecules on CD3^+ T lymphocyte and disease activity of systemic lupus erythematosus

目的通过定量流式细胞术检测系统性红斑狼疮(SLE)患者T淋巴细胞表面Fas受体的分子数,探讨SLE的发病机制及与疾病活动指数的相关性。方法对36例活动期SLE患者和18名正常人群采集外周血,采用Fas定量流式细胞试剂盒荧光染色,通过流式细胞仪对T淋巴细胞上表达Fas受体分子数和凋亡率进行检测。结果活动期SLE患者T细胞表面表达Fas受体分子数和凋亡率较正常人群明显上调(P<0.01)。活动期SLE患者T细胞表面Fas受体分子数与T细胞凋亡率和SLEDA I之间,具有明显的正相关(P<0.01),抗dsDN A抗体阳性患者组T细胞表面Fas受体分子数较抗dsDN A抗体阴性组明显增高,差异具有统计学意义(P<0.05)。结论SLE患者T淋巴细胞表面Fas受体分子数上调,由Fas介导的T淋巴细胞凋亡加快,刺激机体产生抗dsDNA等多种自身抗体,可能是引起SLE免疫功能紊乱的主要原因。SLE患者T淋巴细胞表面Fas受体分子数与SLE的活动性呈正相关,是一较好的评价SLE疾病活动性的指标。

Objective To study the expression of surface Fas receptor molecules on CD3+ T lymphocytes, and to investigate the relationship with pathogenesis and activity of systemic lupus erythematosus (SLE). Methods Dual flow cytometry was used to quantitatively measure the expression of Fas receptor molecules and apoptosis on CD3+ T lymphocytes from 36 active patients with systemic lupus erythematosus and 18 healthy individuals. Results The expression of Fas receptor molecules and apoptosis on CD3+ T lymphocytes of SLE patients were significantly higher than that of healthy group (P<0.01). There was positive correlation between Fas receptor molecule, percentage of CD3+ T lymphocytes and activity index of SLE (P<0.01). On the other hand ,Fas receptor molecule expression on CD3+ T lymphocytes from the patients with positivity anti-dsDNA antibody was high, compared with that of patients with negative anti-dsDNA antibody (P<0.05). Conclusion The results suggest that the expression of Fas receptor molecules on CD3+ T lymphocytes from SLE patients is relatively high. Increased expression of Fas receptor molecules on CD3+ T lymphocytes accelerates the apoptosis of lymphocytes and stimulates the production of antibodies. It is an indicator for disease activity evaluation in SLE patients.