系统性红斑狼疮患者外周血T B淋巴细胞Fas FasL表达及与凋亡的关系研究

Relationship between the expression of Fas or FasL and the apoptosis of peripheral blood T and B lymphocytes in systemic lupus erythematosus patients

目的探讨系统性红斑狼疮(SLE)患者外周血T、B淋巴细胞Fas、FasL表达及与凋亡的关系。方法采用流式细胞术(FCM)检测了30例SLE活动期患者、30例SLE非活动期患者和30名正常人外周血T、B淋巴细胞的凋亡率及Fas、FasL表达率。结果T、B淋巴细胞的Fas表达率依次为:活动期组>非活动期组>正常对照组(P<0.01);FasL的表达率除B淋巴细胞在SLE活动期显著高于正常对照组外(P<0.01),其余各组间差异无统计学意义;细胞的凋亡率在活动期显著高于非活动期(P<0.01),而非活动期与正常对照组差异无统计学意义(P>0.05);SLE的活动性与T或B细胞的Fas、FasL表达率间无相关关系,而与T或B细胞的凋亡率呈正相关(P<0.01);无论是T或B细胞,Fas、FasL的表达率与凋亡率间无相关性。结论SLE患者体内外周血淋巴细胞的凋亡受到多种因素影响,但最终仍表现为凋亡率的异常增高,特别是活动期SLE,推测外周血淋巴细胞凋亡率的增高在SLE发病机制中起着重要作用。

Objective To investigate the relationship between the expression of Fas or FasL and the apoptosis of peripheral blood T and B lymphocytes in systemic lupus erythematosus (SLE) patients. Methods The expression rates of Fas and FasL and apoptosis rates of peripheral blood T and B lymphocytes were detected by flow cytometry (FCM) in 30 active SLE patients and 30 inactive SLE patients and 30 healthy controls. Results There were significant difference in the expression rates of Fas of T and B lymphocytes among three groups (P<0.01), that is, active SLE group was higher than the inactive group and the inactive group was higher than healthy control group. No marked difference was found in FasL expression of lymphocytes among these groups except that the expression rate of FasL in B lymphocytes was higher in active SLE than in controls (P<0.01). Meanwhile, the apoptosis rates of lymphocytes were significantly higher in active SLE than in inactive SLE(P<0.01), but had no difference between inactive SLE and healthy controls (P>0.05). In addition, the activity of SLE had no correlation with the expression rates of Fas or FasL on T or B lymphocytes but has positive correlation with the apoptosis rates of T or B lymphocytes. And no correlation was found between the expression rate of Fas or FasL and the apoptosis rate of neither T nor B lymphocytes. Conclusion The apoptosis of peripheral blood lymphocytes is influenced by many factors in SLE patients, but is abnormally increased at last, especially in active SLE. It is inferred that the increase of apoptosis rate of peripheral blood lymphocytes plays an important role in the pathogenesis of SLE.