建立周围神经二次损伤的实验动物模型

Establishment of an animal model of twice injury of the peripheral nerve

目的建立周围神经二次损伤的动物模型研究神经二次损伤后对周围神经再生的影响。方法3 2只SD大鼠随机分成4组。A组是在第1次损伤2周后修复,术后3周予以第2次损伤和修复;B组是在第1次损伤2周后修复,术后6周再予以第2次损伤和修复;C组是在第1次损伤4周后修复,术后3周予以第2次损伤和修复;D组是在第1次损伤4周后修复,术后6周予以第2次损伤和修复。结果大鼠全部存活,术后A组于2 8～3 6d ,B组于2 4～3 2d ,C组于3 8～45d ,D组于2 7～3 4d后出现左前肢屈曲活动。结论该实验模型可行有效、重复性好,为进一步研究二次损伤对神经再生的影响提供了实验依据。

Objective In order to study the influence of second time injury on nerve regeneration, an animal model of twice injury of the peripheral nerve was established. Methods 32 Sprague Dawley rats were randomized into 4 groups. In group A, the musculocutaneous nerve was transected and then repaired in 2 weeks. The nerve was re-transected 3 weeks following the first repair and re-coapted right away. In group B,the first repair of the nerve was done 2 weeks after the first transection, and the second time transection and repair was done 6 weeks after the first repair. In group C, the first repair was done after 4 weeks of nerve transection, while the second time injury and repair was carried out 3 weeks after the first repair. In group D, the first repair was done in 4 weeks of transection, and the second time injury and repair was done 6 weeks following the first repair. Nerve regeneration following the second time injury and repair was evaluated postoperative by checking elbow flexion. Results All rats survived the operations. Elbow flexion appeared in 28～36 days, 24～32 days, 38～45 days, and 27～34 days following the second repair in groups A, B, C, and D, respectively. Conclusion The procedure is practicable and reproducible. It provides a reliable animal model to study the influence of second time nerve injury on peripheral nerve regeneration.