大鼠胰腺移植后早期病理变化

Pathological changes of pancreatic graft early after its transplantation in rats

目的 观察大鼠胰腺移植后早期病理变化。方法 70只SD大鼠随机分成7组:假手术组(Sham组) ,冷缺血2h组(Ⅰ组) ,冷缺血2h再灌注1、3、6、9、12、2 4h组(I/R 1、3、6、9、12、2 4 )。H -E染色后光镜及电镜观察各组胰腺组织的病理变化。结果 I/R 1、3、6组可观察到细胞凋亡的典型改变。结论 细胞凋亡是胰腺移植后的早期事件,移植胰腺冷缺血再灌注后早期主要的死亡方式是凋亡,而不是坏死。

Objective To investgate the pathological changes of pancreatic graft early after its transplantation in rats. Methods Pancreas transplantation was performed in 7 groups of male SD rats (n=5 pairs each): to one group,sham operation was performed (Sham group), to the ischemic (I) group, the graft was kept cold and ischemic for 2 h before transplantation, to the 5 ischemia-reperfusion (I/R) groups, the graft was pretreated by allowing 2 h of cold ischemia followed by reperfusion for 1 h,3 h,6 h,12 h and 24 h, respectively. The pathologic changes of the graft pancreas tissue were observed under optic and electron microscopes. Results Typical apoptotic cells were found present only in I/R1 h,I/R3 h and I/R6 h groups. Conclusion Apoptosis of graft pancreas cells is an early event after its transplantation. The mode of this cell death is apoptosis, instead of necrosis.